Intern- CPCCG

Job Description

Job Description

The student will have the opportunity to conduct research related to developing assays that utilize patient-specific hiPSC to model the role of noradrenergic neurons of the locus coeruleus (LC) in Alzheimer's disease (AD). The LC, which is the brain's primary source of noradrenaline, is among the earliest regions of the brain to degenerate in AD. This project aims to generate (iPSC)-derived noradrenergic neurons through transcription factor overexpression and then validate the identity through marker expression using qPCR, immunofluoresence, and ELISAs.

Skills and techniques, that the intern will learn to:

• Stem cell culture, cloning, qPCR, immunofluoresence, and ELISAs.

• Become proficient at using instruments and software required for high throughput and high content

screening and analysis.

• Demonstrate flexibility in adapting methods/skills according to evolving project priorities.

• Perform experiments and procedures, demonstrating increasing expertise with a wide range of

techniques.

• Contribute to experimental design and follow up planning, interpretation of results and

evaluation of data.

• Analyze and interpret data from different sources and draw relevant conclusions.

• Learn key aspects of writing reports and be given the opportunity to practice and contribute to those.